|
KMID : 1024420100140030208
|
|
Food Engineering Progress
2010 Volume.14 No. 3 p.208 ~ p.216
|
|
|
Assessment of Applicability of a Calcium Carbonate-Alginate Beads as Neutralizer for the High Cell Density Cultivation of Isolated Sourdough Lactic Acid Bacteria
|
|
Jung Seung-Won
Lee Kwang-Geun
Kim Cheol-Woo
Lee Su-Han
|
|
|
Abstract
|
|
|
|
Lab scale experiments were conducted in order to assess the applicability of CaCO3-alginate beads as neutralizer for the high cell density cultivation and prepare the direct vat inoculation cultures of isolated sourdough lactic acid bacteria. With increasing the amount of bead and decreasing the diameter of bead in acidic solution, the neutralizing effect of CaCO3-alginate bead became higher. In batch process with CaCO3-alginate beads, Lactobacillus amylovorus DU-21 isolated from sourdough showed the highest viable cell counts and optical density in MRS broth. The values of viable cell counts and optical density were 9.996 log CFU/mL and 3.97, respectively. Experiments on the conditions which increase viability during lyophilization were carried out and the following results were obtained; 15% glycerol revealed the high cryoprotective effect on the concentrated cultures during lyophilization among the two cryoprotective agents. Consequently, CaCO3-alginate beads and 15% glycerol were found to be useful not only to cultivate Lactobacillus amylovorus DU-21 but also to preserve strain.
|
|
|
KEYWORD
|
|
|
CaCO3-alginate beads, neutralizer, direct vat inoculation cultures, lactic acid bacteria
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|